Summary: Plant Cell And Tissue Culture Course Guide | Vreugdenhil
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When does calcofluor white show fluorescence?
When bound to specific polysaccharides: cellulose, hemi-cellulose and chitin.
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When is a colouring with calcofluor white used, and why?
To check if walls are completely digested in protoplasts. It's sensitivity is high and binds stable.
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What can be detected by fluphenazine?
Calmodulin, a co-factor that binds to Ca2+.
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What does DAPI stain?
Nuclei in fixed cells. It is water soluble.
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What is necessary to distinguish between DNA and RNA staining by propidium iodide (PI)?
Treatment with nucleases.
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What is a downside of staining with propidium iodide?
It is membrane impermeant and excluded from living cells.
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With which stain can mitochondria be detected?
With DIOC or with MitoTracker Red (both in living cells).
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What is stained with aniline blue?
Callose in cell walls.
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For what is callose staining often used?
To visualize the walls of pollen tubes or sieve pores in phloem of vascular tissue.
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What can be stained with Nile red?
Organic solvents and hydrophilic lipids: intracellular lipid droplets.
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