Cell and Tissue Culture

7 important questions on Cell and Tissue Culture

What is the capacity of single cells to develop into a complete new individual called?

Totipotency.

What is the ability of individual undifferentiated cells to develop into one or several specific cell types called?

Competence.

What does it mean when a competent cell has become comitted?

A competent cell has become fixed or determined to a specific developmental pathway.

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The induction of the determined state has been called ...

permissive induction.

What is the difference between callus and tumour tissue?

Tumour tissue grows as callus but does not require phyto-hormones for it's growth.

The amount of DNA in one genome is expressed in a value. What is this value called?

The c-value.

What is the principle of synchronisation?

To accumulate cells in a specific phase by inhibiting the transfer to the next cell-cycle phase, followed by a release from that inhibition.

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