Protoplast fusion
8 important questions on Protoplast fusion
Through which parent are organelles transmitted in plants?
Through the mother plant.
There are two perequisites for recombination between genomes. What are these?
They can come in physical contact, and sufficient homology at the DNA level should be present.
What puts so many strong restrictions on the parental combinations from protoplast fusion products?
The genetic information of two genotypes has to operate in a strongly co-ordinated way.
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Why can combinations of unrelated species grow as callus, but do not regenerate into plants?
It is assumed that the restrictions for metabolic functions are less severe than for morphogenesis.
How can protoplast fusion achieve the goals of introducing traits from a wild type species in to a cultivated crop plant?
You reduce somatic incongruity problems and you do not increase the ploidy level in the asymmetric hybrids.
How can partial genome transfer take place?
When a micronucleus can be isolated and can be fused with a recipient protoplast.
Partial genome transfer can also take place by ...
micro-injection.
Why is the possibility to obtain novel combinations of organelles using protoplast fusion unique?
Because dure to maternal inheritance of organelles, it is not possible to obtain novel combinations of chloroplats and mitochondria sexually.
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