Gene Transfer and Transgenic Plants
11 important questions on Gene Transfer and Transgenic Plants
How can a transformed cell get a selective advantage?
Genes which confer resistance against compounds that are toxic for wild type cells are introduced together with the gene of interest.
What is the job of a promoter?
It initiates transcription of a gene.
What is direct gene transfer?
The introduction of DNA directly into a plant cell, without the use of a specific plant vector.
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What are the advantages of a direct transfer procedure?
Data are obtained soon after the experiment is done, and it does not require a regeneration system.
What happens during particle bombardment?
Metal particles coated with DNA are rapidly shot into plant tissue.
What is silicon-carbide fibre mediated transformation?
A procedure where the DNA is also introduced into cells with force, this uses small very sharp silicon-carbide needles, which are vortexed into cells. These needle like structures can be mixed with DNA and stick into the cell.
Which traits are analized in a transgenic plant after a transformation experiment?
Number of copies T-DNA, relations between copies, expression of marker used, and expression of genes of interest in introduced T-DNA.
What is the use of activation tagging?
It may identify genes that are normally expressed only after an inducing trigger is given.
What is promoter/enhancer trapping?
Introduction of promoter-less reporter genes into plant genomens, which may result in insertions of reporters downstream of endogenous promoters or enhancer sequences
What are gene traps?
When sequences that act as mRNA splice site acceptors are present just upstream of ORF of reporter gene then reporter may form transcriptional fusion when inserted in a transcribed region.
When does TGS (Transcriptional Gene Silencing) occur?
When genes integrate in a region where the DNA is or easily becomes methylated.
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