Microscopes

7 important questions on Microscopes

What does the resolving power of a lens relate to?

The minimal distance that two dots can still be separated.

How is the resolution of an objective given?

In numerical aperture (N.A.).

What is a microtome?

A machine used for slicing the specimen into thin sections.

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How will electron dense areas appear in a TEM?

Dark, because few electrons pass through.

How many times are specimen stained in preperation for electron microscopy?

Two times.

What is the E face of a plasma mebrane?

The interior face of outer monolayer, because monolayer is on exterior side of membrane.

What are the limitations for Scanning Tunneling Microscopy?

The specimen must be an electrical conductor, and the technique only provides information about electrons associated with the specimens surface.

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