Summary: Powerpoints
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1 Refreshment of molecular biology & Instruction practicum
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1.1 Restriction enzymes
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What is a restriction enzyme?
A protein that recognizes palindromatic DNA sequences -
What are the names of restriction enzymes based on?
The bacteria from which they are isolated. -
What is a characteristic of sticky ends ligation?
It creates an overhang. The overhangs, called "sticky ends", are what allow the vector and insert to bind to each other. -
What is a downside of blunt ends ligation?
It is less stringent than sticky end ligation. -
What do you call enzymes that recognize the same sequence, but are from different organisms?
Isoschizomers . -
What are type I enzymes?
Complex, multisubunit, combination restriction-and-modification enzymes that cut DNA at random far from their recognition sequences. -
What are type IIS enzymes?
Those that cleave outside of their recognition sequence to one side. -
What are type IV enzymes?
Recognize modified, typically methylated DNA. -
1.2 Plasmids
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What is a plasmid?
A small circular double stranded DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. -
What are cloning plasmids used for?
Tofacilitate the cloning of DNA fragments.
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