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  • 1 Refreshment of molecular biology & Instruction practicum

  • 1.1 Restriction enzymes

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  • What is a restriction enzyme?

    A protein that recognizes palindromatic DNA sequences
  • What are the names of restriction enzymes based on?

    The bacteria from which they are isolated.
  • What is a characteristic of sticky ends ligation?

    It creates an overhang. The overhangs, called "sticky ends", are what allow the vector and insert to bind to each other.
  • What is a downside of blunt ends ligation?

    It is less stringent than sticky end ligation.
  • What do you call enzymes that recognize the same sequence, but are from different organisms?

    Isoschizomers.
  • What are type I enzymes?

    Complex, multisubunit, combination restriction-and-modification enzymes that cut DNA at random far from their recognition sequences.
  • What are type IIS enzymes?

    Those that cleave outside of their recognition sequence to one side.
  • What are type IV enzymes?

    Recognize modified, typically methylated DNA.
  • 1.2 Plasmids

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  • What is a plasmid?

    A small circular double stranded DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently.
  • What are cloning plasmids used for?

    To facilitate the cloning of DNA fragments.

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