Strategies of genetic engineering - Tricks with DNA primers
6 important questions on Strategies of genetic engineering - Tricks with DNA primers
Can you join a DNA sticky end with a DNA blunt end?
What is the barrier to prevent the eukaryotic genomic clones/genes from being expressed in bacteria?
What are conventional cloning methods?
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What is nonconventional cloning?
What is TA cloning?
It takes advantage of the 3′ A overhangs added during amplification. These sticky ends enable insertion into a linearized vector with complementary 3′ T overhangs.
TA cloning is used for preparation of gene library. You need to have specific plasmid for insertion.
Taq polymerase adds to 3´ prime deoxyadenosine
What is GC cloning?
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