Strategies of genetic engineering - Introduction and terms

6 important questions on Strategies of genetic engineering - Introduction and terms

What is a Genetically Modified Organism (GMO)

Any organism (except human beings) in which the genetic material has been altered in a way that does not occur naturally by mating or natural recombination or by a combination of both.

What is a transgenic organism?

A subgroup from GMO. An organism containing the gene only from another organism.

What is recombinant DNA?

'Artificial' DNA; DNA from two or more sources is incorporated into a single recombinant molecule.
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What is a recombinant protein?

Protein encoded by the recombinant DNA.

What is molecular cloning?

Preparation of identical copies of DNA fragments.

What is the difference between eukaryotic and prokaryotic cells for gene expression?

Prokaryotic cells don't have posttranslational modification of proteins. Eukaryotic cells do.

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