Genetic Cooking - Vectors
13 important questions on Genetic Cooking - Vectors
What are cloning vectors?
What 3 properties would an ideal cloning vehicle have?
- Low molecular weight.
- Ability to confer readily selectable phenotypic traits on host cells.
- Single sites for a large number of restriction endonucleases.
What are the types of cloning vectors?
- Plasmid
- Phage
- Cosmid
- BAC, YAC
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What is the average size of plasmid vectors?
What 3 things do all plasmid vectors contain?
- Replication Origin (ORI) sequence.
- A gene that permits selection.
- Multiple Cloning Site (MCS): for insertion of exogenous DNA.
What is the structure of a phage vector?
What is a phage vector?
What is a cosmid vector?
What is a BAC?
What is a YAC?
What is a shuttle vector?
What is the plasmid copy number?
What is plasmid incompatibility?
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