Content - Gel electrophoresis
3 important questions on Content - Gel electrophoresis
What for can you use gel electrophoresis?
- To identify the identity of plasmid after digestion.
- For isolation of genes amplified with PCR.
- For isolation of cut plasmid in order to clone the gene inside the plasmid.
- To check whether cloning was successful.
- To check whether PCR worked.
- To check whether cloning was successful after colony PCR.
What do you check when you do a restriction analysis?
Whether the gene has been inserted.
Whether the gene has been inserted in correct orientation.
Whether the gene has been inserted in correct orientation.
What is colony PCR?
Bacterial colonies can be tested for the presence of the plasmid without plasmid isolation, using PCR.
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