Content - Plasmids

6 important questions on Content - Plasmids

What plasmids can you use for reducing the expression of an endogenous gene?

Gene knock-down plasmids.

What are reporter plasmids used for?

For studying the function of genetic elements. These plasmids contain a reporter gene that offers a read-out of the activity of the genetic element.

What are viral plasmids?

These plasmids use technology evolved by viruses to aid in delivery of genetic material into target cells.
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What is the AmpR gene?

Gene for the resistance against ampicillin. Bacteria bearing this plasmid will be resistant.

What is the PuroR gene?

Gene for puromycin N-acetyltransferase causing resistance of transfected cells to puromycin.

What is the Multiple Cloning Site (MCS)?

A DNA region within a plasmid that contains multiple unique restriction enzyme cut sites. There you can easily insert the gene.

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