Genetic Cooking - Promoters

5 important questions on Genetic Cooking - Promoters

What is a constitutive promoter?

A promoter that is not influenced by external factors.

What is a tissue specific promoter?

These direct the expression of a gene in specific tissue(s) or at certain stages of development.

What is an inducible promoter?

Their performance is conditioned to external stimuli that can be artificially controlled. They are usually in bacterial cells.
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What is the U6 promoter used for?

Usually used for shorter transcript, so it’s commonly used for sgRNA.

What would be the best vector choice for cloning the gene and producing the high amounts of protein in bacteria?

Expression plasmid with inducible promoter.

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