RNAi - Mechanism of action

15 important questions on RNAi - Mechanism of action

What strand is the plus strand?

5' - 3'

What strand is the coding strand?

5' - 3'

What is the non-template strand?

5' - 3'
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What is the template strand?

3' - 5'

What is the antisense strand?

3' - 5'

What is the non-coding strand?

3' - 5'

What is the minus strand?

3' - 5'

In what is shRNA (short hairpin RNA) processed?

miRNA (microRNA).

What is the passenger strand?

5' - 3'

What is the guide strand?

3' - 5'

What is the function of the argonaut protein in RISC?

It unwinds the passenger strand of siRNA/miRNA and it cleaves the guide strand.

What strand is the sense strand?

5' - 3'

What is the function of dsRNA?

To be cleaved into little pieces that will stop the translation of mRNA.

What is the function of the enzyme Dicer?

It cleaves dsRNA/ pre-miRNA/ shRNA into 20-25 bp long siRNA and miRNA (in cytoplasm).

What is the function of guide RNA (antisense strand) of siRNA?

Binds to mRNA. mRNA is degraded and no translation and protein production is happening.

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