Genetics has provided a powerful new approach to biological research
8 important questions on Genetics has provided a powerful new approach to biological research
What is reverse genetics?
ATTEMPT TO FIND THAT FUNCTION
2. One approach: completely knoch out the gene's function by eliminating the gene and then to look for the effect on the organism's function
What is DNA cloning?
What is the main goal of probing?
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What are the 3-5 steps of southern blotting?
2. Fragment of SNA get separated into groups of fragments of different sizes by using electrophoresis
3. Seperated fragments are blotted onto a piece of porous membrane
4. The membrane with DNA strands is heated (denaturation) to get single stranded DNA
5. The membrane is placed in a solution of the probe
6. The single stranded probe will find and bind to its complementary DNA sequence
What is western blot?
2. Blotted onto a membrane
3. Membrane is bathed in a solution of antibody
4. Position of the protein is revealed by the postion of the label that the antibody carries
What is a microarray?
What are the (3) steps during the microarray method?
--> probe: complex mixture made by converting mRNA from one tissue (for example cancer tissue) into cDNA
2. Spots of label on the glass reveal which genes were being transcribed in the sample tissue
--> the pattern of colours of the microarray 'holes' reveal which genes are actively transcribed in that cell type
3. Comparison with noncancerous tissue reveals which genes are active (and inactive)
What is the main goal of the Polymerase Chain Reaction?
The question on the page originate from the summary of the following study material:
- A unique study and practice tool
- Never study anything twice again
- Get the grades you hope for
- 100% sure, 100% understanding
