Embryo transfer - steps in embryo transfer

7 important questions on Embryo transfer - steps in embryo transfer

When is non-surgically collection of embryos in cattle and horses possible?

it is only possible for embryos that are in the uterus

When is surgical collection of embryos used?

when the embryos are still in the oviducts or in the tip of the uterine horns

What are the 2 methods used by surgical oviduct collection?

1. flushing from the uterine tip towards the fimbria -> not in horses and pigs
1. flushing from the fimbria to the utero-tubal junction
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What is the OPU-technique (ovum pick up) and in which animals is it used?

follicle aspiration under ultrasound guidance, the ultrasound probe is combined with a needle in the vagina

What is the right order of the 7 stages of embryo development (morula, blastocyst etc)?

stage 1, 1 cell
stage 2,  2 cells, 4-8 cells, 16 cells
stage 3, early morula
stage 4, compact morula

stage 5, early blastocyst

stage 6, blastocyst

stage 7, expanded blastocyst

How doe surgical transfers of embryos work?

a pipette in inserted through the fimbria into the ampulla

When is pregnancy rate and embryo survival the same and in which animals?

its the same if only 1 embryo is transferred, in cattle and horses

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