Quantification of stress responses
7 important questions on Quantification of stress responses
What are the steps you need to take in a quantification procedure?
- Data collection
- Model selection
- Quantification of effects
What can we say about the effect of salt on B. cereus?
What can you say about the different strains?
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What can you say about the form of the inactivation?
What are the selection criteria for a model?
- Model can be fitted to all conditions
- Biological meaning of the parameters (CFU, reconcilable parameters)
- Model is flexible: reduction of model parameters (model should fit all different conditions)
What are the things that have to be taken into account for the model of the 2 b cereus strains?
- The model has to take into account that there are 2 strains, the sensitive vegatitive cells and the resistant vegatative cells for the ATCC14579 and the sensitive vegatative cells and resistant spores fore the ATCC10987 strain.
- Effect of salt addaptation on resistance
- The shoulder that is present in some of the slat ATCC14579
What do the things in this model stand for?
N(t), N(0), k(sens), k(res), t, f, t(s)
N(0): Initial concentration
k(sens): inactivation rate sensitive micro organism
k(res): Inactivation rate more resistant micro organism
t: time
f: fraction resistant cells.
t(s): holder period
The question on the page originate from the summary of the following study material:
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