Behavior in Sedentary and Running CTSB KO Mice

10 important questions on Behavior in Sedentary and Running CTSB KO Mice

How were the control mice housed?

With 7-9 in a cage!

What were the results of the Spatial memory (Morris Water Maze) task?

WT-R mice preferred the platform quadrant way more at both 24 and 48hrs, whereas the WT-S only preferred at 24hrs.

Which group showed the most increase in DCX cells?

WT-R mice in both type C and type D
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What was the main difference between CTSB KO mice and WT mice?

Ko mice showed reduced inhibitory neurotransmission, resting membrane potential was more depolarized.

S CTSB able to cross the Blood Brain Barrier?

Yes it is, about 10% of blood CTSB was found in whole brain tissue after 15mins of injection.

What type of cells were investigated in their reaction to CTSB?

adult neural progenitor cells

Which genes were upregulated by exogenous CTSB treatment?

DCX and BDNF

What was the effect of CTSB on hippocampal P11?

P11 increased which is associated with
  • higher DCX gene expression
  • higher brain cholesterol absorption
  • more PC12 cell migration

What was the effect of exercise training for 4 months in humans on CTSB levels?

It increased significantly in the blood and is somewhat positively correlated with VO2 changes (r = .44)

Was there any relation between Complex Figure making and Aerobic fitness?

Yes! a small but significant one

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