Reprogramming
10 important questions on Reprogramming
What are advantages of human ESCs derived from in vitro fertilization?
What is promised to be able to study with human ESCs?
- Study embryogenesis
- Drug screening
- Human diseases models
- Personalized regenerative medicine
How are human SCNT-ESCs generated?
This is technically challenging and requires oocytes.
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What are the 4 transcription factors needed for the generation of mouse iPSCs?
How were the 4 transcription factors needed for generation of iPSCs discovered?
A promoter/enhancer upsteam of e.g GFP -->
If GFP stays off; gene is not active
If GFP is on; gene is active
Reporter assays are used for high-throughput screening
What are the sequential events of reprogramming:
- Initiation
- Maturation
- Stabilisation (iPSCs)
What are two ways to characterize human iPSCs?
- Cell morphology --> differentiation capacity in vivo
- expression of pluripotency genes
How can differentiation of Human iPSCs capacity be checked in vivo?
How can differentiation of human iPSCs capacity be checked in vitro?
- checking for pluripotency- & germ-layer-specific markers.
What are different models of generating iPSCs?
- By introducing the four key transcription factors via:
- Retroviral and lentiviral transduction (integration)
- Sendai viral transduction (non-integration)
- episomal transfection (plasmids)
- Introduction of proteins
- Low molecular weight molecules --> inducing endogenous key transcription factors
The question on the page originate from the summary of the following study material:
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