PCC-CALUX

10 important questions on PCC-CALUX

What are polyhalogenated aromatic hydrocarbons (PHAHs)?

Hydrocarbon means a hydrogen and a carbon molecule.
Aromatic means a ring of 6 carbon atoms
Polyhalogenated means that there are one or more halogen atoms in the ring.

What are the three groups of chlorinated PHAHs?

  • PCDDs
  • PCDFs
  • PCBs

What are practical uses of PHAHs?

  • Agent Orange during the US/Vietnam war
  • Chemical factory in Italy in 1916 (the dioxins were released in the air)
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How can the levels of PHAHs be determined?

  • Extraction of lipid fraction
  • Clean-up
  • Gas chromatography

What is the risk assessment of PHAHs?

The Tolerable Daily Intake (TDI).

During the DR-CALUX bioassay, what happens during cell culturing?

PHAHs bind to Ah-receptor, which binds then to DRE and the cells start producing luciferase.

What is the exposure medium for DR-CALUX?

DMSO

What is remarkable for DMSO?

DMSO is a remarkable organic solvent because it has polar and non-polar characteristics

Why is the amount of light measured?

The amount of light directly depends on the dioxin-like potency of the mixture to which the cells have been exposed.

How is the activity of luciferase measured?

To measure the activity of luciferases cells are lysed so the luciferase enzyme has easy acces to the luciferine substrate that is added.

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