Gene expression: the technology
9 important questions on Gene expression: the technology
GRO-seq (Global Run-On Sequencing)
DNase I hypersensitive site mapping
Chip-Seq (Chromatin Immunoprecipitation)
- Crosslink DNA + bound protein
- Fragment DNA
- Immunoprecipiate
- Release DNA (washing)
- Prepare library
- Sequence
- Map sequence tags to genome and identify peaks
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DCas9 fused to Biotin Ligase
Tools for measuring the transcriptome
- RNA-Seq (Single cell)
- GRO-Seq
Tools for finding the regulatory sites of the genome
- DNase I mapping
- ATAC-Seq (Single cell)
Tools for finding the Genomic Binding Site of a given (modified) protein
- ChIP-seq
- CUT&RUN (ChIC-Seq) (Single cell)
Mapping the 3D Genome
- 3C, 4C
- Hi-C (Single cell)
Identifying the proteins associated with a given genomic site
- APEX
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